Glass @ MindSay

My daughter and I made this parrot.  It took 3 days (to finish) because we have to wait for parts of it to dry.  First, you have to trace a pattern on the oval "glass" (really plastic) with black outline.  You wait till it dries.  Then you can fill in the opening with the color.  Then you wait for that to dry, and voila', you got art!

I then used photoshop to sample the glass and made the Epic-Fu glass.

We decided to go shopping before the zoo, my sister and I!  What a great day we had planned.  And it was going so well!  Out of the six major Thrift World Stores in the Metro area of Omaha, we hit two.  The two nicest ones with the Brand Name clothes!  One on the Northwest side of Omaha and one on the Southeast side of Omaha near the Zoo.

 

What a great way to end our mini camping trip that just turned in to being a mini stay at my sister's house due to the varying weather that kept popping up!  Instead of staying at the really nice free camp grounds we stayed at my sister's house for a few days, I wasn't going to chance being caught near a big man made lake that had extermly high levels near the banks of the camp ground!

 

Anyway, after a GREAT haul of a summer woredrobe for both kids from two different thrift shops and then treating the kids to lunch, we headed to Henery Dooley (sp?) Zoo for a nice afternoon.  It started out great!  We hit the Jungle, the Sea Lions, the Garden of Senses, the Cat House, part of the Avaiary, the Gorrila House, the Cat Complex, the feeding time for the smaller tropical birds, the Desert Dome, and the Giaraff House.  With smaller enclousers along the way of course!  Of course pictures are on here!  Enjoy!

 

While we were just ouside of the New Butterfly House and the Aquareme deciding which one we were going to go in first before we started heading to the town were my van was sitting so the kids and I could head home and my sister and her kids could head home, we heard the tornado sirins go off!  Now I know why so many ppl get killed in public places during storms!  The tornado sirns go off and the majority of the Zoo visitors head for the Goddess Dayum parking lots!  I kid you not!  Well being country girls, my sister and I herd the kids to the aquarememe (sp?) house under the canapoy and bust out our cell phones.  Each of us respectively calling our hubby's, asking them why the hell neither one of them called to tell us there was a storm system heading straight at Omaha!  We informed both of them that we were heading for the storm shelters in the Fish house and we would call once the storm passed.  Then we hearded the kids towards one of the Zoo Workers holding a door open to the basement of the complex leading to the exective offices and we were one of the first groups down. 

 

There were a few other ppl down there already but what we found the most funny out of this was that everyone single person down there including us, were NOT from Omaha persay but from the surrounding communities and areas!  We are NOT fools!  While the main secruity guy was telling us and the few zoo workers what the plan was, he was getting reports from the weather radio and the few of us that stopped and made calls.  Then he asked us to sit tight, while he and his other crew went outside to go round up the rest of the Zoo visitors and get them inside various zoo complexes till the storm passed.

 

We had a couple of upset kids of course, my daughter being one of them, and a lot of upset ppl, including ball players that stuck around after the College World Series before they went home.  Those of us from the midwest were laughing and joking and calming the kids down.  We kept telling everyone that we are in one of the safest buildings and don't worry about the animals they know what to do and the zoo workers were making sure they were safe also.  Once all the zoo workers got in the main secruity guy came back in to our basement and said it was pitch black out and winds up to 90-100 miles per hour and they would not know if a tornado hit till after it was clear out.  The lights did go out and thankfull the generator kicked on right away.

 

After we got the okay to clear out and leave the zoo, we got some pictures of the damage done in side the zoo.  Down trees and one of the heavy glass safety entrance doors to the Wild Kingdom Palliviallion was completely blown off and laid gently down in front of the Pavillion!  I missed that picture! There was hail everywhere!  We had to make our way out of the Eastern Side of Omaha back towards the I-10 exit so we could head to Freemont NE which is an hour Northeast of Omaha, where my van was sitting!  Damage everywhere!  While driving the news said, the Art Festival got hit hard downtown Omaha, there was a boat taken off of a dock in the Missiouri and was dropped on the roof of a house, trees blocking roads, and down electrical poles all over Omaha downtown/metro area!  We stopped on the outskirts of town to feed the kids and the Taco Johns we hit had running TVS.  The area heading to Fremont NE was hit hard also and a semi rig was entangled in electrical wires!  Cars of the road, rigs off the road, and major damage.  This system worked its way into IA and massive damage and two killed on the IA side.

 

We got detoured outside of a town between Omaha and Fremont due to massive damage and accidents.  Where I got a phone call from Randy when he got home.  My dog Spud died during the day yesterday!  Randy was upset and of course the kids and I were very upset also, but we also knew the dog was going to end up dying on us.  Spud was the dog with the unknown seizures.  At first Randy thought he somehow choked while he was outside on the chain but when he went up to examine Spud, there was plenty of slack in the chain and the collar would have slipped right off of his neck (we made sure that it could slip off if he was caught up when running or on the chain if something would have happened).  The way Spud was laying and his facial features, you could tell he had a massive siezure.  So trying to find our way on back country roads and county paved hiways we didn't know to get back into Fremont, we had upset kids and nobody was out directing the traffic.  My sister and I again being country girls said fuck it and got on a country road and started heading towards the sun because Northwest was where we wanted to head.  We picked up a truck in front of us from our home area just a different county and he had the same idea.  We got rerouted via the roads with no help from anyone due to big trees, silos, fencing, and other debries in the mushy gravel roads!  A normal 1 hour drive from Omaha to Fremont took 2 hours!

 

Once we got to a lil town Southeast of Fremont which would have been the quickest route we got denied by a State Trooper directing traffic.  Nobody was allowed into town where a small lake was surrounded wiht houses and such due to the damage!  They weren't letting anyone through not even family members of the town or land owners with no houses.  Ambulances and Fire trucks were all over the town and two of the rural fire trucks were making their way towards the other road leading to Fremont.  We just turned around and followed the fire trucks into Fremont.  Finally!  Fremont NE had no major damage.  Some trees down and some windows broken but no major damage.  Then we pull up into the truck stop where we left my van.  I got a lovely suprise!  My rear windsheild was blown out or a rock got kicked up in the storm!  I have no rear windshield now!  Nothing was stolen.  But everything was glass covered clear up to the front seat and everything was water logged!  Thank GODS I had what ever paperwork I did have in there in sacks and covered up!  My Leadership Manual alone would have cost me 100 some dollars to replace if it got water logged or damaged somehow!

 

So I called Randy yet again and told him we FINALLY made it into Fremont but I would be still a lot later because we had to clean up the glass and find places for the kdis to sit with out getting cut up!  Thankfull we were able to pull a couple of the pillows from underneith my tent that dind't have glass on it and give those to the kids to sit on.  My sister handed over one of her towels so I could sit on it while driving.  And then we rearrange the back so none of my Thrift shop finds, my tents, sleeping bags, blankets, pillows, or other camping gear would go flying out for my hour drive home!  I ended up pulling over twice on the way home to rearrange my back but FINALLY my normal two hour drive ended up being a 4 hour and 45 minute drive and we made it home at 8:45pm.  where we promptly unloaded the van and shop vaccued it out so I could get some plastic on the windshield due to more storms coming this weekend.

 

After we got that done, we gave Spud my Fat Man Dog a burial worthy of a King.  See we couldn't bury him.  The ground is too wet and his smell would draw the local wild dog pack and the local coyote pack.  Not to mention all the other wild critters int he area.  So we took my lovely burn pile Randy made up for me this weekend and gave Spud a Funeral Pry.  We didn't want any other anmals digging him up and eating on him.  Randy loaded him on the bonfire and I threw some sage, cedar chips, and sweet grass all over Spud and we said a lil blessing for him and said our goodbyes.

 

Now today, I get to call his old family and inform them that he is gone.  I am doing laundry from the back of my van and going to take the kids down to my girl's house because they are still stressed from last night.  A lil fun down at Aunties house and coming home about the time Daddy gets home from work will do them good. 

 

Now I am off to throw another sleeping bag into the wash!  Pictures to come after this post!

Many cultures obtained from a culture collection, such as ECACC, will arrive frozen and in order to use them the cells must be thawed and put into culture. It is vital to thaw cells correctly in order to maintain the viability of the culture and enable the culture to recover more quickly. Some cryoprotectants, such as DMSO, are toxic above 4oC therefore it is essential that cultures are thawed quickly and diluted in culture medium to minimize the toxic effects.

A schematic diagram of "Resuscitation of Frozen Cell Lines"

Materials

• Media– pre-warmed to the appropriate temperature (refer to the ECACC Cell Line Data Sheet for the correct medium and size of flask to resuscitation into.)
• 70% ethanol in water
• DMSO

Equipment

• Personal protective equipment (sterile gloves, Laboratory coat, safety visor)
• Waterbath set to appropriate temperature
• Glass Bottom Dishes
• Microbiological safety cabinet at appropriate containment level
• CO2 incubator
• Pre labeled flasks
• Marker Pen
• Pipettes
• ELISA plates
• Ampule Rack
• Tissue

Procedure

1. Read Technical data sheet to establish specific requirements for your cell line.
2. Prepare the flasks as appropriate (information on technical data sheet). Label with cell line name, passage number and date.
3. Collect ampule of cells from liquid nitrogen storage wearing appropriate protective equipment and transfer to laboratory in a sealed container.
4. Still wearing protective clothing, remove ampule from container and place in a waterbath at an appropriate temperature for your cell line e.g. 37oC for mammalian cells. Submerge only the lower half of the ampule. Allow to thaw until a small amount of ice remains in the vial - usually 1-2 minutes. Transfer to class II safety cabinet.
5. Wipe the outside of the ampule with a tissue moistened (not excessively) with 70% alcohol hold tissue over ampule to loosen lid.
6. Slowly, dropwise, pipette cells into pre-warmed growth medium to dilute out the DMSO (cell culture flasks prepared in Step 2).
7. Incubate at the appropriate temperature for species and appropriate concentration of CO2 in atmosphere.
8. Examine cells microscopically (phase contrast) after 24 hours and sub-culture as necessary.

Key Points

1. Most text books recommend washing the thawed cells in media to remove the cryoprotectant. This is only necessary if the cryoprotectant is known to have an adverse effect on the cells. In such cases the cells should be washed in media before being added to their final culture flasks. See Protocol 7 for further details.
2. Do not use an incubator to thaw cell cultures since the rate of thawing achieved is too slow resulting in a loss of viability.
3. If a CO2 incubator is not available gas the flasks for 1-2 minutes with 5% CO2 in 95% air filtered through a 0.25m filter.
4. For some cultures it is necessary to subculture before confluence is reached in order to maintain their characteristics e.g. the contact inhibition of NIH 3T3 (Prod. No. 93061524) cells is lost if they are allowed to reach confluence repeatedly.

Scouce: ECACC Handbook Protocol 2

Dixie currently feels:

Jealous

 

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This blog is for my fellow bespectacled counterparts.

All of the specky, visually impaired, short-sighted, blind-as-a-bat, four-eyed geeks like myself.

 

All of those with 20/20 vision, or contact lenses - you won't understand.

 

Of course, wearing glasses gives you the impression that you're intelligent.

The nerds of the world spend their time sat at computers, reading books, playing video games - our eyes are damaged, so we're forced to wear these discs of refracting glass on our faces.

 

And, there's the bonus of being able to look straight ahead in a blizzard or gale - and not having to squint.

 

But then, there's the walking in the rain.

- Glasses don't come with windscreen wipers, and they SHOULD.

 

There's the swimming.

- It's pretty damn impossible to see the other side of the pool without them, for most, so stay out of our lane.

 

There's the general rough and tumble of the day.

- I've had mine knocked off my face by about four different clumsy people now.

The first one happened the day after I got them!

 

And of course, you'd have to remove them in a mosh pit.

I've knocked mine off myself many a time by forgetting to take them off before I slammed on a bit of Rammstein.

 

 

But don't forget - the worst possible thing about glasses...

 

LOSING THEM ON YOUR HEAD.

 

Seriously!

The world all laugh - when we're looking for them, they're balanced on our head.

 

"Where are they?" - we wail.

"I can't see without them!" - we panic.

 

Eventually, we're told that they're on our head.

And that's when you feel like an idiot - and you may even blush.

 

Mine steam up when I blush.

As well as coming in from the cold weather outside - there! Another downside! Steaming up!

 

 

For all of you non-bespectacled people...

When they're on our head... We don't feel them!

 

They're very light. They're designed to be.

They're comfortable too - they get fitted to the shape of our heads.

 

It's just like when you're wearing a cap, or a hairband. You don't realise it's there after a while.

 

Spare us the humiliation and the panic, two-eyed people of the world.

 

Just tell us where they are, please.

 

 

 

"Hmmm... I don't know! Where DID I put them...?"

Tony's care blew up today. He has first and second degree burns.

 

 

And no one could even say, "Wow, what a shame."

 

But you could all stop in and gawk at the pictures.

 

I'm disappointed in you.